Separation and Purification of Prim-O-glucosylcimifugin and 5-O-methylvisammioside from Saposhnikovia Divaricata by High-speed Counter-current Chromatography
- VernacularTitle:高速逆流色谱分离纯化防风中升麻素苷和5-O-甲基维斯阿米醇苷
- Author:
Zhongming HAN
;
Yunhe WANG
;
Mei HAN
;
Shujie ZHAO
;
Limin YANG
- Publication Type:Journal Article
- Keywords:
Counter-current chromatography;
Saposhnikovia divaricata;
Prim-O-glucosyl-cimifugin;
5-0-methylvisammioside
- From:
Chinese Journal of Analytical Chemistry
2009;37(11):1679-1682
- CountryChina
- Language:Chinese
-
Abstract:
High-speed counter-current chromatographic ( HSCCC ) method was successfully used to separate and purify prim-O-glucosylcimifugin and 5-0-methylvisammioside from Saposhnikovia divaricata(Thicz. ) Schis-chk. ,a traditional Chinese herb,with a solvent system composed of ethyl acetate-n-butanol-water(2:7:9,V/V). The lower phase of the system was used as the mobile phase at the flow rate of 2.0 mL/min,and the upper phase was used as the stationary phase. The separation produced a total 13.9 mg prim-O-glucosylcimifugin and 25.0 mg 5-0-methylvisammioside with the purity of 98. 1% and 99.2% determined by high performance liquid chromatography (HPLC) from 316 mg of crude sample from S. divaricata. The structures of isolated compounds were identified by ESI-MS,~1H NMR and ~(13)C NMR.
