Determination of Zeranols in Plant Tissue by Rapid Resolution Liquid Chromatography-Mass Spectrometry/Mass Spectrometry
- VernacularTitle:快速高分离液相色谱-串联质谱法测定植物组织中雌激素玉米赤霉醇类化合物
- Author:
Yan ZHANG
;
Hui LI
;
Jingxuan ZHANG
;
Pin Lü
;
Lixia WANG
;
Jingze LIU
- Publication Type:Journal Article
- Keywords:
Rapid resolution liquid chromatography-mass spectrometry/mass spectrometry;
plant tissues;
zeranols
- From:
Chinese Journal of Analytical Chemistry
2009;37(11):1683-1686
- CountryChina
- Language:Chinese
-
Abstract:
A method was developed for the determination of zeranols (α,β-zearalanol,α,β-zearalenol,zear-alanone,zearalenone) with RRLC-MS/MS in the plant tissue. The samples were extracted with acetonitrile and reextracted with aqueous alkaline solution,cleaned up with MAX column and then determined by RRLC-MS/MS using multiple reaction monitoring ( MRM) scan mode. The results showed that the working curves were linear in the range of 0 -20 μg/kg. The limits of detection ( LOD) of zeranols were 0.5 μg/kg and the limits of quantification (LOQ) was 1. 0μg/kg. Extraction recoveries for zeranols ranged from 75. 8% to 105.4% and RSD was between 2.4% and 12.1%. The method is suitable for the determination of zeranols in the plant tissue.
