Simple and Efficient Enantioseparation of Ofloxacin by Capillary Electrophoresis with Contactless Conductivity Detedction Using Binary Chiral Selectors
- VernacularTitle:毛细管电泳/非接触式电导法分离检测氧氟沙星对映体
- Author:
Dan LIU
;
Ruixia WEI
;
Tianyao XIE
- Publication Type:Journal Article
- Keywords:
Capactively coupled contactless conductivity detection;
capillary electrophoresis;
chiral separation;
binary chiral selectors;
ofloxacin
- From:
Chinese Journal of Analytical Chemistry
2009;37(11):1687-1690
- CountryChina
- Language:Chinese
-
Abstract:
Novel method of enantioseparation of ofloxacin by capillary electrophoresis with capactively coupled contactless conductivity detection (C~4D) using binary chiral selectors,hydroxypropyl cyclodextrin and hydroxypropyl methyl cellulose ( HP-β-CD and HPMC) ,was developed. The enantiomers of ofloxacin were baseline separated with an uncoated fused-silica capillary(45 cm×50 m i. d. ,L_(effi) = 40 cm)under the optimum separate conditions, 20 mmol/L HAc +6 mmol/L NaAc + 12 mg/L HPMC +35 mmol/L HP-β-CD,separation voltage +13 kV,electrokinetic injection 11 kV×10 s. The calibration curve of the enantiomers showed good linearity in the range from 0. 8 mg/L to 40 mg/L with LOD =0. 3 mg/L. Effects of several factors on resolutions,such as the composition of the running buffer,the pH value,separation voltage,injection method and the parameters of C~4D detector,were investigated. The efficacy of the HP-β-CD and HPMC were discussed. The proposed method has been successfully applied to monitor the enantiomer of ofloxacin contents in the commercial Ofloacin Tablets and Levofloxacin Hydrochloride Tablets,and was demonstrated simple,rapid and reproducible.
