Immunoglobin G Antibodies to Purified-Protein-Derivative and Lipoarabinomannan-B by Enzyme-Linked Immunosorbent Assay in the Diagnosis of Tuberculous Pleural Effusion.
10.4046/trd.1995.42.4.455
- Author:
Tai Hoon MOON
1
;
Chul Ho CHO
;
Seung Min KWAK
;
Jin Ju KIM
;
Sang Nae CHO
Author Information
1. Department of Internal Medicine, Inha University College of Medicine, Sungnam, Korea.
- Publication Type:Original Article
- Keywords:
Tuberculous pleural effusion;
PPD;
LAM-B;
ELISA
- MeSH:
Antibodies*;
Diagnosis*;
Enzyme-Linked Immunosorbent Assay*;
Humans;
Immunoglobulin G;
Pleural Effusion*;
Sensitivity and Specificity;
Skin Tests;
Tuberculosis;
Tuberculosis, Pulmonary
- From:Tuberculosis and Respiratory Diseases
1995;42(4):455-464
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Considering that both humoral and cell mediated immunities play an important role for human tuberculosis infection, enzyme-linked immunosorbent assay(ELISA) measurement of immunoglobulin G (IgG) antibody to mycobacterial antigens can be used for the serologic diagnosis of tuberculous pleural effusion. METHOD: We measured absorbance values of IgG antibodies to purified-protein-derivative (PPD) and lipoarabinomannan-B (LAM-B) in the pleural fluid (PF) and the serum in 40 tuberculous (TPE) and 19 nontuberculous pleural effusions (NTPE). RESULTS: 1) The IgG antibodies to PPD and LAM-B were significantly (P<0.0005) higher in the PF and the serum of TPE compared to NTPE. 2) The IgG antibodies to PPD and LAM-B in the serum were higher than that in PF. 3) Significant correlations were found between pleural and serum IgG antibodies to PPD and LAM-B. 4) With a cutoff value for IgG antibody to PPD in the PF of 0.091, sensitivity was 55.0% and specificity 94.7% in the diagnosis of TPE. 5) With a cutoff value for IgG antibody to LAM-B in the PF of 0.337, sensitivity was 50.0% and specificity 94.7% in the diagnosis of TPE. 6) The seropositive rates in TPE were not related to PPD skin test status, the amount of PF and coexisting active pulmonary tuberculosis. CONCLUSION: The assay of IgG antibodies to PPD and LAM-B might be useful for the diagnosis of TPE. Our study suggests the mechanism of passive transfer of IgG antibodies to PPD and LAM-B from the serum to the PF through pleural tissue.
