Determination of methomyl in rat plasma by SPE-HPLC
- VernacularTitle:SPE-HPLC法分析大鼠血浆中灭多威
- Author:
Pengfei ZHAO
;
Bailin LI
- Publication Type:Journal Article
- Keywords:
forensic toxicological analysis;
methomyl;
plasma;
SPE;
HPLC
- From:
Chinese Journal of Forensic Medicine
2009;24(6):386-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of solid phase extraction-reversed-phase high performance liquid chromatography(SPE-HPLC)for determination of methomyl in plasma of rat.Methods The sample pretreatment method,the test conditions,the linear range,the sensitivity,the specificity,the precision, the accuracy,the stability and the recoveries for plasma were investigated by using rat plasma spiked with standard methomyl and intemal standard substance.Results The linear range was 0.1～20μg/mL ( r= 0.9993,P<0.001).The limit of detection was 0.03μg/mL(S/N ≥3).The intra and inter-day precision of assay for methomyl was less than 8.33%and 11.11%in plasma respectively.The intra and inter-day accuracy of assay for methomyl was between 90%and 120%in plasma respectively.The recoveries for methomyl were more than 88%±4.4%in plasma.Conclusion The HPLC method for quantitative and qualitative analysis of methomyl is simple,rapid and accurate,which is suitable for the identification of methomyl in the cases.
