Application of a new magnetic beads made in China in DNA extraction of forensic biological samples with automation workstation
- VernacularTitle:国产磁珠结合自动化工作站批量提取生物检材DNA的应用
- Author:
Dian YANG
;
Hong LIU
;
Chao LIU
;
Qingyu WU
;
Yue LI
;
Changhui LIU
;
Wenli MA
- Publication Type:Journal Article
- Keywords:
forensic biological evidence;
automatic DNA workstation;
DNA quantitation;
muhiplex STR amplification
- From:
Chinese Journal of Forensic Medicine
2009;24(6):404-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the application of a new magnetic beads made in China in DNA extraction of forensic biological samples with automation workstation.Methods DNA was extracted from common forensic biological samples by QIAGEN Bio-Robert Universal System and a new magnetic beads made in China,and then typed with Identifiler system in ABI3130XL Genetic Analyzer.210 of these samples were also quantitated by ABI7500 Real Time System.Results Total of 9100 genomic DNA was extracted from various forensic biological samples by the new magnetic beads made in China and automation workstation methods,and most of them were successfully typed for STR analysis.In these biological samples,oral swabs and muscles were of the highest Success rate of STR typing(100%),and the lowest was touched cell samples (50.0%).Conclusion The new magnetic beads made in China with automation workstation methods can be applied to DNA extraction of most forensic biological samples.
