Expression and significance of cytochrome C and Bax in hippocampus of posttraumatic stress disorder rat
10.3969/j.issn.0529-1356.2009.06.004
- VernacularTitle:细胞色素C和Bax在创伤后应激障碍大鼠海马神经元的表达及意义
- Author:
Xiaoming LI
;
Fang HAN
;
Yuxiu SHI
- Publication Type:Journal Article
- Keywords:
Posttraumatic stress disorder;
Cytochrome C;
Bax;
Immunohistochemistry;
Laser scanning confocal microscopy;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2009;40(6):869-872
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of cytochrome C (Cyt C)and apoptosis-related gene Bax in hippocampus of posttraumatic stress disorder(PTSD)rats in order to investigate their effects and relationships. Methods Sixty male Wistar rats were used in the present study. The single-prolonged stress(SPS)-method was carried out to set up the rat PTSD models. There were six groups:after SPS 1,4,7,14,28 days groups and control group. The expression of Cyt C and Bax proteins were detected with immunohistochemistry, double immunofluorescence, confocal laser scanning microscopy and Western blotting. Results The expression of Cyt C peaked at 4 days and maintained higher level at 7 days after SPS. At 7 days after SPS, strong immunoreactivity of Bax was noticed,At 14 days after SPS, the expression of Bax was down-regulated and then gradually decreased. Conclusion Bax is upregulated and promotes the releasing of Cyt C. And the releasing of Cyt C may be one of mechanisms of inducing apoptosis in the hippocampus of PTSD rats.
