Effect of fibrinogen gel and chitin on BMSCs to chondrocytes differentiation
10.3969/j.issn.0529-1356.2009.06.012
- VernacularTitle:纤维蛋白凝胶与几丁质对骨髓间充质干细胞向软骨细胞分化的影响
- Author:
Dongmei LI
;
Lianhong JIN
;
Baodong ZHANG
;
Shuiyou YANG
;
Jicheng LI
- Publication Type:Journal Article
- Keywords:
Bone-marrow-derived mesenchymal stem cells;
Chondrocyte;
Fibrinogen gel;
Chitin;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2009;40(6):909-913
- CountryChina
- Language:Chinese
-
Abstract:
Objective We compare the effects of fibrinogen gel and chitin on BMSCs to chondrocytes differentiation in order to explore the relationship between three-dimensional scaffold and cartilage tissue engineering seed cells BMSCs differentiation. Methods BMSCs together with chitin and fibrin gel complexes were cultured in vitro and implanted into rat's articular cartilage defect location. After 14 days in vitro culture, HE staining, toluidine blue staining and type II collagen immunohistochemical staining were performed;After transplantation in vivo, for 2 weeks, 4 weeks and 6 weeks, morphology observations, expression of cartilage-specific protein analysis and BMSCs in vivo tracer method were performed. We analyzed the differentiation of BMSCs into chondrocytes by statistical methods. Results The comparison of positive cell rate of type II collagen immunohistochemical staining in BMSCs-fibrin gel group and BMSCs-chitin group which were cultured in vitro, showed no significant difference with control group. Integrated absorbance(IA) change rate of toluidine blue staining and type II collagen immunohistochemical staining in BMSCs-fibrin gel group which was cultured in vivo, showed significantly different with other groups and control group.Conclusion The results showed that in vitro fibrin gel or chitin has very weak induction of BMSCs to cartilage differentiation, while in vivo BMSCs-fibrin gel can facilitrate BMSCs to differentiate into chondrocyte-like cells.
