Effect of Grp78 on the activation and expression of ERK 1/2 in human hepatocellular carcinoma tissues
10.3969/j.issn.0529-1356.2009.06.016
- VernacularTitle:Grp78表达对人肝细胞癌细胞外信号调节激酶1/2活性和表达的影响
- Author:
Rongjian SU
;
Zhen LI
;
Liufang CHENG
;
Hongdan LI
;
Jia WEI
;
Cuifen BAO
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma;
Grp78;
ERK1/2;
siRNA;
SMMC-7721;
Immunohistochemistry;
Western blotting;
Human
- From:
Acta Anatomica Sinica
2009;40(6):928-932
- CountryChina
- Language:Chinese
-
Abstract:
Objective We examined the Grp78, ERK1/2 and phospho-ERK1/2 expressions in hepatocellular carcinoma(HCC) tissue samples in vitro, we interfered the expression of Grp78 in SMMC-7721 cells to explore whether Grp78 is involved in ERK1/2 signal pathway. Methods The Grp78, ERK1/2 and phospho-ERK1/2 expressions were detected by immunohistochemistry and confirmed by Western blotting in 47 HCC tissue samples. The Grp78 expression in SMMC-7721 cells was interfered by plasmid transfection and siRNA, ERK1/2 phosphorylation and expression were determined by Western blotting. Results The Grp78 expression was significantly correlated with ERK1/2 and phospho-ERK1/2 in HCC tissue samples. Overexpression of Grp78 promoted ERK1/2 phosphorylation in SMMC-7721 cells and the increased ERK1/2 phosphorylation was inhibited by Grp78 knockdown. Conclusion Grp78 is involved in the regulation of ERK1/2 signal pathway and might be a potential target for the comprehensive therapy of HCC.
