Distributions of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa
10.3969/j.issn.0529-1356.2009.06.032
- VernacularTitle:大鼠咽黏膜内ATP受体P2X_3阳性感觉纤维的分布
- Author:
Zhaojin WANG
;
Yanqing WANG
- Publication Type:Journal Article
- Keywords:
Purinergic receptor;
Calcitonin gene-related peptide;
Afferent fiber;
Pharyngeal mucosa;
Immunofluoresenthistochmistry;
Rat
- From:
Acta Anatomica Sinica
2009;40(6):1005-1007
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the distribution of purinergic P2X_3 receptor immunoreactive afferent fibers in the rat pharyngeal mucosa and provide morphological evidence for exploring the role of ATP in the signal transduction of sensory stimuli. Methods Twelve adult Wistar rats were used and immunofluorescenthistochemical double-labeling technique combined laser confocal scanning microscope was applied in the present study. Results 1.P2X_3 immunoreactive fibers were observed on sections from all parts of pharyngeal mucosa. Two types of positive fibers were found. One was free nerve fibers covered with many varicosities. Another one ramified in mucosa and showed complex arborization endings. Nerve plexus in mucosa were formed by P2X_3 immunoreactive fiber ramifications. 2.Most calcitonin gene-related peptide (CGRP) positive fibers intermingled with P2X_3 immunoreactive fiber arborizations. A few P2X_3 immunoreactive fibers were covered with many varicosities co-localized with CGRP. 3.In petrosal ganglion, most neurons were stained with P2X_3 or CGRP immunoreactivity and small number of P2X_3 immunoreactive neurons co-stained with CGRP. Conclusion These results indicated that the different types of afferent fibers in rat pharyngeal mucosa expressed purinergic P2X_3 receptor immunoreactivity and ATP might be related to nociceptive or physiological signals transduction in rat pharyngeal mucosa.
