Protective and therapeutic effects of Sagittara Sagittifolia L. Xiao-Zhi Pills on nonalcoholic steatohepatitis in rats
- VernacularTitle:慈菇消脂丸对大鼠非酒精性脂肪性肝炎的防治作用
- Author:
Yanhua MA
;
Shaojun YANG
;
Jianxiong ZHAO
- Publication Type:Journal Article
- Keywords:
nonalcoholic steatohepatitis;
Sagittara Sagittifolia L. Xiao-Zhi Pills;
cyclooxygenase-2;
inducible nitric oxide synthase;
nitric oxide
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2009;30(6):754-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the preventive and therapeutic effects of Sagittara Sagittifolia L. Xiao-Zhi Pills on nonalcoholic steatohepatitis (NASH) in rats. Methods Rat NASH models were established and the rats were divided randomly into five groups: the normal group, model group, high-dosage group and low-dosage groups of the treatment, and Dongbao Gantai positive drug group. Histopathological changes of the liver were observed. The levels of MDA, superoxide dismutase (SOD), and NO (nitric oxide) in the liver homogenate were determined. Expressions of cyclooxygenase-2 (cox-2) and inducible nitric oxide synthase (iNOS) in the liver were determined with immunohistochemical technique. Results In model group, extensive adipose degeneration and inflammatory cell infiltration were found in the liver. Compared with those in normal group, SOD activities in model group were obviously decreased (P<0.01), the contents of MDA and NO were obviously increased (P<0.01), and the expressions of cox-2 and iNOS proteins were obviously increased (P<0.01). Compared with those in model group, the activities of SOD in treatment groups were obviously increased (P<0.01), the contents of MDA and NO were obviously decreased (P<0.01), and the expressions of COX-2 and iNOS proteins were obviously decreased (P<0.01). The effect of high-dosage group was more obvious than that of Dongbao Gantai group (P<0.05). Conclusion Sagittara Sagittifolia L. Xiao-Zhi Pills can effectively prevent and treat nonalcoholic steatohepatitis in rats, and its mechanism may be associated with ameliorating hepatocellular steatosis, removing the free radicals and enhancing the anti-oxidation and anti-inflammation capability.
