High-efficiency gene transfer into rabbit smooth muscle cells by pseudotyped retroviral vector
- VernacularTitle:假型逆转录病毒载体高效介导兔平滑肌细胞基因转染
- Author:
Fei PEI
;
Rui HE
;
Junyan LI
;
Hong YU
- Publication Type:Journal Article
- Keywords:
pseudotyped retroviral vector;
smooth muscle cell;
gene transfer
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2009;30(6):766-768
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct pseudotyped retroviral vector MuLV/VSV-G and transfer it into rabbit smooth muscle cells (SMC) in order to provide a high-efficiency vector for SMC gene transfer. Methods We constructed pseudotyped retroviral vector MuLV/VSV-G containing the previously reported gene lacZ, determined the titer, and determined the efficiency of gene transfer into SMC mediated by pseudotyped retroviral vector MuLV/VSV-G. Finally the transfer efficiency was compared with that by MuLV. Results MuLV/VSV-G vector was constructed. The titer of the vector was 6-7.8×10~6CFU, the transfer efficiency was (92±12)% by using MuLV/VSV-G vector and (24±5)% by MuLV vector. Conclusion Pseudotyped retroviral vector MuLV/VSV-G which was constructed successfully is a kind of high-efficiency gene transfer vector in smooth muscle cells.
