The Effect of rh-endostatin on Phenotype of HUVECs at Different Activating Levels

VernacularTitle:恩度对不同状态内皮细胞表面标记物影响的实验研究
Author: Yan LIU ; Chuangui ZHANG ; Kai LI
Publication Type:Journal Article
Keywords: Human umbilical vein endothelial cells; Endostar; CD105; CD62E
From: Chinese Journal of Clinical Oncology 2009;36(23):1370-1373
CountryChina
Language:Chinese
Abstract: Objective: In order to reveal the mechanisms of rh-endostatin and its suppression effect on HUVECs at different activating levels. Methods: Three types of HUVEC were prepared. We detected the ex-pression of CD105 and CD62E on the surface of adhering HUVEC, HUVEC of exponential phase of growth, and HUVEC activated with TNF-alpha. The effect of endostatin on these three types of HUVEC was indicated by the variation in the expression of CD105 and CD62E on the cells. The relationship of the changes in cell markers with the time and the concentration of endostatin was analyzed by SPSS 11.5. Results: The expres-sion of CD105 was correlated with time and dose of endostar in all groups. The expression of CD62E was cor-related with the dose of endostar in HUVEC treated with TNF-α for 24h. Conclusion: Endostatin of a certain dose can inhibit the activation of endothelial cells but it cannot induce cell apoptosis.