Effects of different doses of topiramate on expression of NCAM and GAP- 43 mRNA in hippocampus of rats with epilepsy
- VernacularTitle:不同剂量托吡酯对癫大鼠海马组织中NCAM和GAP- 43 mRNA表达的影响
- Author:
Danhong WU
;
Ping ZHONG
;
Guanshui BAO
;
Hong ZHOU
;
Jun SHEN
;
Feifei HUANG
- Publication Type:Journal Article
- Keywords:
epilepsy;
topiramate;
nerve cell adhesion molecule;
growth-associated protein 43
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(12):1459-1462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different doses of topiramate (TPM) on the expression of nerve cell adhesion molecule (NCAM) and growth-associated protein 43 (GAP- 43) mRNA in hippocampus of rats with epilepsy. MethodsForty-eight rats were randomly divided into normal control group, kainic acid (KA) group, 10 mg/kg TPM group, 40 mg/kg TPM group, 100 mg/kg TPM group and 400 mg/kg TPM group (n=8). The models of rats with epilepsy treated by different doses of TPM were established. The behavior of rats was observed, and the expression of NCAM and GAP- 43 mRNA in hippocampus of rats was determined by Real-time PCR. Results The expression of NCAM and GAP- 43 mRNA in KA group was significantly higher than that in normal control group (P<0.01), while there was no significant difference between 10 and 40 mg/kg TPM groups and KA group, that in 100 and 400 mg/kg TPM groups was significantly lower than that in KA group (P<0.01), and that in 400 mg/kg TPM group was significantly lower than that in 100 mg/kg TPM group (P<0.01). Conclusion KA can up-regulate the expression of NCAM and GAP- 43 mRNA in hippocampus of rats with epilepsy. Higher dose of TPM can inhibit the expression of NCAM and GAP- 43 mRNA, and the inhibitory effect is related with the dose of TPM.
