Effect of TFB on TGF-β1 signaling pathway in HSC of liver fibrosis rats
- VernacularTitle:鬼针草总黄酮对肝纤维化大鼠肝星状细胞TGF-β1信号传导通路的影响
- Author:
Liping YUAN
;
Feihu CHEN
;
Ling LU
;
Pengfei DU
;
Bo HU
;
Fanrong WU
;
Fenglai YUAN
;
Jun LI
- Publication Type:Journal Article
- Keywords:
total flavonoids of Bidens Bipinnata L(TFB);
hepatic stellate cells (HSCs);
TGF-β1 signaling pathway;
hepatic fibrosis;
proliferation;
smad
- From:
Chinese Pharmacological Bulletin
2009;25(12):1655-1659
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the proliferation of HSCs stimulated by exogenous TGF-β1 (transforming growth factor betal),observe the effect of TFB(total flavonoids of Bidens Bipinnata L.)on smad2/7,typeⅠcollagen mRNA and protein expression of HSCs and study the protective effect and molecular mechanism of TFB on hepatic fibrosis.Methods HSCs were isolated with collagenase Ⅳ perfusion in situ and density gradient centrifugation. The effect of TFB on cell proliferation was observed by MTT colormetric assay. The auto-secretion of TGF-β1 and synthesis of type Ⅰ collagen were measured by enzyme-linked immuneadsordent assay (ELISA).Moreover,the expression of smad2/7, typeⅠcollagen mRNA and protein was measured by semi-quantitative RT-PCR and Western blot methods respectively.Results TFB could markedly inhibit the proliferation of HSCs of liver fibrosis rats stimulated by TGF-β1 and production of TGF-β1 and type Ⅰ collagen.In addition,TFB treatment could significantly down-regulate smad2 and type Ⅰ collagen mRNA expression and up-regulated smad7 mRNA expression of HSCs Smad2 protein expression of HSCs stimulated by TGF-β1 was also down-regulated by TFB.Conclusion TFB has the protective effect against hepatic fibrosis by inhibiting the activation of TGF-β1 signaling pathway and suppressing the HSC proliferation.
