Anticancer effects of a novel indolinone compound in nasopharyngeal carcinoma cells

VernacularTitle:一种新型吲哚满酮类化合物对鼻咽癌细胞的作用
Author: Mingqun WANG ; Yinan LIU ; Xiang ZHAO ; Ye ZHANG
Publication Type:Journal Article
Keywords: Antineoplastic agents; Nasopharyngeal neoplasms; Cell cycle; Cell adhesion
From: Journal of Peking University(Health Sciences) 2009;41(6):640-644
CountryChina
Language:Chinese
Abstract: Objective: To develop novel targeted anticancer medicines for effective treatment of nasopharyngeal carcinoma ( NPC) , a prevalent malignant disease in southern China and southeast Asia.Methods: CNE cells were treated with a novel indolinone IF239 synthesized by our research group. Cell viability was determined by the acid phosphatase assay ( APA). Morphologic changes and adhesion status of CNE cells treated with IF239 were observed under a light microscope. Flow cytometry was used to analyze the cell cycle phases . Key regulating molecules in the cell cycle progression were detected by Western blotting. Results: IF239 had potent cytotoxic effect on CNE cells. The possible antitumor mechanisms of IF239 involved inhibition of cell adhesion and cell cycle arrest in the G2_/M phase. Moreover,G2_/M arrest caused by IF239 was related to up-regulation of both cyclin B1 and the phosphorylation level of CDK1. Conclusion: IF239 has high anticancer activity over CNE cells, and has unique anticancer mechanisms, suggesting that IF239 has promising application potentials.