Effects of dehydroepiandrosterone on amino acids receptor subunit NR2B and GBR1 expressed in primary cultured rat cerebral cortical neurons
- VernacularTitle:脱氢表雄酮对大鼠大脑皮质神经元氨基酸受体亚单位NR2B和GBR1表达的影响
- Author:
Gai XUE
;
Honghai WU
;
Yanning HOU
- Publication Type:Journal Article
- Keywords:
dehydroepiandrosterone;
amino acids receptor;
NR2B;
GBR1;
cerebral cortex;
neuron;
neurosteroid
- From:
Chinese Pharmacological Bulletin
2009;25(12):1602-1605
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of dehydroepiandrosterone(DHEA)on the levels of NR2B and GBR1 expressed in primary cultured rat cerebral cortical neurons.Methods Primary cultured rat cerebral cortical neurons were treated with DHEA of different concentrations (1,10,100 μmol·L~(-1))and the expression of amino acids receptor subunit NR2B and GBR1 were detected by immunocytochemistry.Results Compared with control group,the expression intensity of NR2B increased by 15.6%,19.9% and 49.4% after DHEA-L,DHEA-M and DHEA-H treatment(P<0.05 or P<0.01);the expression intensity of GBR1 increased by 14.5% and 58.5% after DHEA-M and DHEA-H treatment(P<0.05 or P<0.01).Conclusion DHEA can enhance the expression of neuron receptor subunit NR2B and GBR1.
