Inhibition effect of China cobra venom active factor on endothelial cells and its biochemical mechanism
- VernacularTitle:眼镜蛇毒活性组分抑制内皮细胞生长及其生化机制初探
- Author:
Liu ZHU
;
Qingsheng YU
;
Mu YUAN
;
Xinyan LIU
;
Guiping WANG
;
Honge YU
;
Xiaohua LOU
;
Maikun TENG
- Publication Type:Journal Article
- Keywords:
snake venom;
China cobra venom;
endothelial cells;
cytoskeleton;
nitrogen oxide
- From:
Chinese Journal of Biochemical Pharmaceutics
2009;30(6):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To study the effect of China cobra venom active factor(CCVAF) from China cobra venom on endothelial cells and its mechanism.Methods MTT experiment was adopted to evaluate the effect of CCVAF on bovine arteria pulmonalis vascular endothelial cells(BAVEC).The Eosin-Coomassie brillient blue and rhodamine-phalloidin method was used for actin cytoskeleton.Flow cytometry for [Ca~(2+)]_i and spectrophotometry were used for lactate dehydrogenase(LDH) and nitrogen oxide(NO) levels in cell culture supernatant.Results CCVAF(0.625-20 μg/mL) inhibited the proliferation of BAVEC in dose-dependent manner,and IC50 of CCVAF on BAVEC was 2.45 μg/mL. After CCVAF and BAVEC coincubation, it was showed that regression of intercellular conjunctions and disorder of F-actin distribution occurred. The content of [Ca~(2+)]_i, [LDH] and [NO] increased respectively.Conclusion CCVAF can inhibit BAVEC proliferation and it maybe associated with the change of cytoskeleton and increasing of [Ca~(2+)]_i,[LDH] aod [NO].
