Study on pharmacokinetics of genioside and breviscapine in rats in Zhideng injection
- VernacularTitle:栀灯注射液中栀子苷、灯盏花素的大鼠药代动力学初步研究
- Author:
Zhongliang LIANG
;
Zhenzhou JIANG
;
Luyong ZHANG
;
Yong QIN
;
Rongrong WANG
;
Jing LIU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Zhideng injection;
breviscapine;
genioside;
pharmacokinetics
- From:
Chinese Journal of Biochemical Pharmaceutics
2009;30(6):371-374
- CountryChina
- Language:Chinese
-
Abstract:
Purpose A RP-HPLC method was used to determine genioside and breviscapine in plasma and to study its pharmacokinetics in rat, respectively.Methods Rat plasma samples were collected after a single dose of Zhideng injection and pharmacokinetic parameters of genioside and breviscapine were estimated,respectively.Results A good linear relationship was obtained between 0.2-40.0 μg/mL for breviscapine, and 0.5-200.0 μg/mL for genioside.The recoveries from plasma were larger than 85%,and RSDs of inter-day asaay and intra-day assay were below 10%. The pharmacokinetic results showed that genioside and breviscapine were rapidly eliminated from plasma after iv administration of three doses of Zhideng injection.The mean half-life was 72.6 min and 21.6 min,respectively.Conclusion The established HPLC method was suitable for the pharmacokinetic study of genioside and breviscapine.
