Apoptosis of K-562 cells induced by oleum curcumae wenchowensis and its mechanisms
- VernacularTitle:莪术油诱导K-562细胞凋亡分子机制的实验研究
- Author:
Jia LIU
;
Qun SHEN
;
Jianmin JI
;
Guangrong ZHU
;
Yacheng ZHANG
;
Pengjun JIANG
;
Xiaochao SUN
- Publication Type:Journal Article
- Keywords:
oleum curcumae wenchowensis(OCW);
K-562 cell line;
apoptosis;
Fas/FasL
- From:
Chinese Journal of Biochemical Pharmaceutics
2009;30(6):390-392
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effects and the mechanism of oleum curcumae wenchowensis (OCW) with different concentrations (0, 2.5, 5, 10 and 20 mg/mL) on chronic myeloid leukemia cell line K-562 cells in vitro.Methods The apoptosis of K-562 cells was dyed by Hoechest 33258 and detected by flow cytometry marked with Annexin V/PI. The Expression of Fas/FasL, bcr/abl, bcl-2 and p53 was detected by semi-quantity reverse transcript-polymerase chain reaction (RT-PCR) and Western blot.Results The results showed that the apoptosis rates were gradually elevated. The expression of Fas and FasL protein was increased in a concentration dependent manner, while bcr/abl, bcl-2 and p53 had no significant changes.Conclusion OCW could induce the apoptosis of K-562 cells by up-regulating the expression of Fas/FasL protein.
