Small interfering RNA suppresses the increase of myocyte enhancer factor 2A expression in PC12 cells exposed to hypoxia
- VernacularTitle:小干扰RNA对缺氧PC12细胞中肌细胞增强因子2A蛋白表达的影响
- Author:
Jing XU
;
Mei XUE
;
Jiqing CHEN
;
Chao LU
;
Guoping ZHOU
;
Yan ZHOU
- Publication Type:Journal Article
- Keywords:
small interfering RNA;
myocyte enhancer factor 2A;
PC12 cells
- From:
Chinese Journal of Biochemical Pharmaceutics
2009;30(6):400-403
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the effect of small interfering RNA on the increase of myocyte enhancer factor 2A(MEF2A) expression in PC12 cells exposed to hypoxia.Methods PC12 cells were cultured under normal conditions or were exposed to hypoxic conditions.Small interfering RNA targeted MEF2A gene (MEF2A-siRNA) was chemically synthesized. Eukaryocytic expression vector was built and transfected into PC12 cells with liposome. The expression of MEF2A mRNA was detected by real-time PCR. Western blot was used to detect the MEF2A protein.Results Compared with normal control(2~(-△△CT)=1.01±0.02), the mRNA level of MEF2A gene in PC12 cells with the treatment of MEF2A-siRNA was down-regulated significantly by 88%(2~(-△△CT)=0.12±0.03, P＜0.01).The expression of MEF2A protein in hypoxia-treated PC12 cells was markedly higher than that of normal control(98.4±11.7 and 47.5±7.6,P＜0.01).However, MEF2A-siRNA could significantly suppress the increase of MEF2A protein (P＜0.01).Conclusion MEF2A gene silence induced by siRNA might inhibit the increase of MEF2A protein by hypoxia in PC12 cells.
