Expression change of stromal cell-derived factor 1α in retinas after partial optic nerve injury
- VernacularTitle:视神经部分损伤后视网膜基质细胞衍生因子-1α表达的变化
- Author:
Dongchao PAN
;
Yongyan BI
;
Dongfu FENG
;
Ertao CHEN
;
Shenghua CHU
;
Yang WANG
- Publication Type:Journal Article
- Keywords:
optic nerve injury;
retina;
stromal cell-derived factor 1α
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2009;29(12):1424-1427
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of expression of stromal cell-derived factor 1α (SDF-1α) in retinas after partial optic nerve injury in rats. Methods Models with injury of partial optic nerve were induced in rats. Retinal tissues were collected 1,2,3,5,7,10 and 14 d after injury. Enzyme linked immunosorbent assay and Real-time quantitative PCR were employed to detect the expression of SDF-1α protein and mRNA in retinal tissues respectively in injury group (n=28), sham operated group (n=28) and normal control group (n=12). Results The expression of SDF-1α protein and mRNA in retinas was higher than that in sham operated group and normal control group at different time points after injury (P<0.01), and it reached the peak at the 5th day after injury. The expression of SDF-1α protein and mRNA maintained a high level at the 14th day after injury. Conclusion The expression of SDF-1α protein and mRNA is up-regulated after partial optic nerve injury, and may last for a long time.
