The screening and polymorphic analysis of bovine tetranucleotide STR loci
- VernacularTitle:牛四核苷酸STR基因座的筛选和多态性分析
- Author:
Aiping CHEN
;
Xiaoyan MA
;
Hongyu SUN
- Publication Type:Journal Article
- Keywords:
forensic biological evidence;
tetranucleotide STR locus;
genome screening
- From:
Chinese Journal of Forensic Medicine
2009;24(6):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen the microsatellites with low occurrence rate of stutter band and establish the effective bovine STR typing system.Methods The tetranucleotide STR loci in bovine genome were searched with Tandem Repeat Finder software.Primers were designed and used to amplify these candidate loci and the PCR products were separated with electrophoresis.DNA samples from 100 head of unrelated cattle were typed.Results Among these candidate loci,6 bovine tetranucleotide STR loci showed high polymorphism,and their CDP and CPE value were 0.99995 and 0.859591 respectively.Conclusion The 6 bovine tetranucleotide STR loci can be used for bovine identification and parentage testing.
