Accelerated cardiac remodeling of post-infarction was associated with changes of gene expression profile in untreated streptozotocin-induced diabetic rats
- VernacularTitle:STZ诱导的糖尿病大鼠急性心肌梗死后心脏重构及相关基因的表达
- Author:
Guangyuan SONG
;
Ximei WANG
;
Yuejin YANG
;
Hongliang ZHANG
;
Hanjun PEI
;
Zhenyan ZHAO
;
Yongjian WU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Myocardial infarction;
Left ventricular remodeling;
Microarray analysis
- From:
Chinese Journal of Pathophysiology
2009;25(12):2302-2309
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the time-dependent effects of diabetes mellitus (DM) on the development of cardiac remodeling in untreated streptozotocin (STZ)-induced rats with acute myocardial infarction (MI). METHODS: The left anterior descending coronary arteries were ligated 10 weeks after DM induction without any therapy. Transmission electron microscopy, echocardiography, heart weight to tibial length ratios, histological examination, microarray analysis, and real time-PCR were utilized to monitor the changes up to 56 d. RESULTS: After MI, the diabetic rats experienced lower survival rate compared to non-diabetic animals. The pathophysiologic changes indicated that DM accelerated the cardiac remodeling post-infarction. In primary examination, 164 genes related to cardiac remodeling were found to be candidates for hierarchical analysis, such as leucine-rich PPR-motif containing (interleukin-6 signaling pathway), procollagen type I and III, fibronectin-1, RT1, and TIMP-1, etc. The gene expression profile at 14 d in diabetic rats were comparably similar to both 14 d and 28 d in non-diabetic rats, while such changes at 28 d and 56 d in diabetic rats was also similar to the ones at 56 d in non-diabetic rats. CONCLUSION: The accelerated cardiac remodeling of post-infarction in STZ-induced untreated diabetic rats seems be associated with the different profile of gene expressions.
