Effects of Snail1 siRNA on tubular epithelial-to-mesenchymal transition induced by high glucose
- VernacularTitle:Snail1 siRNA 对高糖诱导肾小管上皮细胞表型转变的影响
- Author:
Kaiyun FANG
;
Mingjuan SHI
;
Ying XIAO
;
Huazhen GUI
;
Bing GUO
;
Guozhong ZHANG
- Publication Type:Journal Article
- Keywords:
Snail1 siRNA;
Kidney tubules epithelial-to-mesenchymal transition;
High concentration of glucose
- From:
Chinese Journal of Pathophysiology
2009;25(12):2424-2429
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of Snail1 siRNA on high-glucose induced tubular epithelial-to-mesenchymal transition (TEMT). METHODS: Subconfluent renal tubular epithelial cells were incubated in serum-free DMEM for 24 h to arrest and synchronize the cell growth. Then cells were treated with normal glucose (5.5 mmol/L D-glucose) or high glucose (25 mmol/L D-glucose) for 72 h. Meanwhile 19.5 mmol/L D-manntiol was used as high osmotic control. Snail1 siRNA was transfected into tubular epithelial cells. In parallel, cells were transfected with non-specific siRNA which served as the control data sets. Cells were then treated with 25 mmol/L D-glucose for 72 h. RNA and cell lysates were collected to determine the protein and mRNA levels of Snail1, TGF-β_1, α-SMA, vimentin and E-cadherin. RESULTS: Transfection caused the decreases in Snail1 at mRNA and protein levels by 62% and 68% respectively as compared to those in untransfected cells cultured in high glucose medium. Western blotting exhibited that Snail1 siRNA transfection restored E-cadherin protein expression by 61% compared to that in high-glucose-treatment cells, whereas it inhibited high-glucose-induced induction of α-SMA protein by 58%. Similarly, RT-PCR revealed that Snail1 siRNA transfection dramatically suppressed the high-glucose-induced mRNA expressions of α-SMA and vimentin by 72% and 61%, respectively, while E-cadherin mRNA increased by 53%. CONCLUSION: Our study provides direct evidence that Snail1 is able to control TEMT.
