E1A inhibits growth and increases radiosensitivity of tumors in nude mice implanted with nasopharygeal carcinoma cells
10.3872/j.issn.1007-385X.2009.06.012
- VernacularTitle:E1A抑制鼻咽癌细胞裸鼠移植瘤的生长并增强其对放疗的敏感性
- Author:
Huaping XIAO
;
Rongrong ZHOU
;
Yuping LIAO
- Publication Type:Journal Article
- Keywords:
E1A gene;
nasopharygeal neoplasms;
P53 gene;
radiotherapy
- From:
Chinese Journal of Cancer Biotherapy
2009;16(6):614-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effect of E1A gene on the growth of tumors in nude mice implanted with nasopharygeal carcinoma CNE2 cells and its promotion effect on the racliosensitivity of CNE2-implanted tumors, and to investigate the related mechanism. Methods: E1A gene was transfected into CNE2 cells using adenovirus system, and sta-ble E1A positive clones were established. The inhibitory effect of E1A on tumor formation-ability of CNE2 cells was ob-served in nude mice. The efficacy of E1A gene therapy with or without radiotherapy against CNE2 cell-implanted tumors was evaluated. The effect of E1A gene therapy on the expression of P53 was detected by RT-PCR. Results: CNE2 cells stably transfected with E1A gene (CNE2-Ad-E1A) were successfully established. The tumor formation time was later and tumor size was smaller in CNE2-Ad-E1A cell-implanted mice compared with those in CNE2 cell- and CNE2-Ad-β-gal cell-implanted mice (CNE2 cells stably transfected with Ad-β-gal). Radiotherapy, E1A gene therapy and E1A gene + radio-therapy all suppressed the growth of implanted tumors, with the tumor suppression rates being (60.32±5.34) %, (70.53±6.12) %, and (97.15±4.87) % , respectively. E1A gene therapy significantly increased the expression of P53 gene in tumor tissues. Conclusion: E1A can inhibit the growth of tumors in mice implanted with nasopharygeal carcinoma cells, and enhance its sensitivity to radiotherapy, which may be related to the increased expression of P53 gene in tumor tissues.
