Preparation of PEI-RGD/~(125)I-(α_v)ASODN and its inhibitory effect on invasive ability of HepG2 cells
10.3872/j.issn.1007-385X.2009.06.011
- VernacularTitle:PEI-RGD/~(125)I-(α_v)ASODN的制备及其对肝癌HepG2细胞侵袭力的抑制
- Author:
Haidong CAI
;
Yu QIAO
;
Xueyu YUAN
;
Yuehua YANG
;
Shidong YUAN
;
Ming SUN
;
Zhongwei LV
- Publication Type:Journal Article
- Keywords:
polyethyleneimine-Arg-Gly-Asp (PEI-RGD);
integrin α_v;
antisense oligodeoxynueleotide (ASODN);
hepatocellular carcinoma;
invasion
- From:
Chinese Journal of Cancer Biotherapy
2009;16(6):609-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of ~(125)I-(α_v)ASODN on the in vitro invasive ability of heptocellular carcino-ma cell line(HepG2) through PEI-RGD-mediated receptor process. Methods: Intergrin α_v-specific antisense oligonucle-otide was labeled with ~(125)I, and PEI-RGD/~(125)I-(α_v)ASODN complex was prepared by combining ~(125)I-(α_v)ASODN with polyethyleneimine derivative PEI-RGD. PEI-RGD/~(125)I-(α_v)ASODN complex was transferred into HepG2 cells through the receptor-mediated process. The effect of PEI-RGD/~(125)I-(α_v)ASODN complex on the invasive ability of HepG2 cells was examined by Boyden chamber invasive assay. Results: (1) The labeling yield and radiochemical purity of ~(125)I-(α_v) ASODN were(73.78±4.09)% and(96.68±1.38)%, respectively, and the labeled compound had a good stability in vitro after 48 h at 37℃; (2) The ability of HepG2 cells to uptake PEI-RGD/~(125)I-(α_v)ASODN reached its peek ([12.77±0.85] % ) when PEI-RGD/~(125)I-(α_v)ASODN was at 4 μl/2 μg ([12.77±0.85] %), and then gredually decreased thereafter. So the dosage of PEI-RGD/~(125)I-(α_v)ASODN for the following experiment was chosen as 2 μl/1 μg; (3) The invasive capacity of HepG2 cells was significantly reduced in PEI-RGD/~(125)I-(α_v)ASODN group compared with those in other experiment and control groups (P <0.01 ). Conclusion: ~(125)I-(α_v)ASODN mediated by PEI-RGD can effectively inhibit the invasive capacity of HepG2 cells.
