Effect of Mannan Peptide Combined with 5-Fu on the Proliferation and Apoptosis of HepG2 Cells
10.3969/j.issn.1000-8179.2010.01.011
- VernacularTitle:甘露聚糖肽联合5-氟脲嘧啶对HepG2细胞增殖及凋亡的影响
- Author:
Rongrong ZHAO
;
Liting ZHANG
;
Hong CHEN
- Publication Type:Journal Article
- Keywords:
Mannan Peptide;
5-Fu;
HepG2 cells;
Cell apoptosis;
Cell cycle
- From:
Chinese Journal of Clinical Oncology
2010;37(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of Mannan Peptide combined with 5-Fu on the proliferation and apoptosis of HepG2 cells and to explore the related mechanism.Methods:HepG2 cells were divided into four groups:the control group,5-Fu group,Mannan Peptide group and combination group.Cell proliferation was evaluated by MTT assay.Flow cytometry was used to examine the cell cycle,proliferation index(PI)and apoptosis ratio.The expression of Bcl-2 protein in each group was analyzed by immunocytochemical method.Results:The inhibitive effect of 5-Fu plus Mannan Peptide was significantly stronger than that of Mannan Peptide alone,in a time-and dose-dependent manner. Cells were treated with medicine for 48 hours.Compared with cells in the control group and 5-Fu group,the percentage of cells in G_0/G_1 stage and the apoptosis ratio in the combination group were increased(P<0.01).In immunocytochemical analysis,the combination of Mannan Peptide and 5-Fu could significantly decrease Bcl-2 protein level(P<0.01).Conclusion:The combination of Mannan Peptide and 5-Fu can synergistically suppress the proliferation and induce apoptosis of human hepatoma HepG2 cells.The mechanism might be associated with down-regulation of Bcl-2 protein expression.
