Expression of Stem Cell Marker in Side Population Cells Isolated from Human Gallbladder Carcinoma Cell Line GBC-SD
10.3969/j.issn.1000-8179.2010.01.012
- VernacularTitle:人胆囊癌SP细胞的分离及其干细胞标记物的表达
- Author:
Jun HU
;
Jianzhong LIU
;
Xin YUE
;
Jian LI
;
Renyi QIN
- Publication Type:Journal Article
- Keywords:
Gallbladder neoplasms;
SP cells;
Tumor stem cells;
Flow cytometry
- From:
Chinese Journal of Clinical Oncology
2010;37(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the existence of side population cells with the potency of stem cells in human gallbladder carcinoma cell line GBC-SD and the differences in ABCG2,Oct-4 and CD34 expression among SP cells,non-SP cells and GBC-SD cells.Methods:SP and non-SP cells were sorted from GBC-SD cells by fluorescence-activated cell sorting(FACS).The expression of ABCG2,Oct-4 and CD34 in SP cells,non-SP cells,and GBC-SD cells was detected by reverse transcription-polymerase chain reaction(RT-PCR),Western blot,flow cytometry(FCM)and immunofluorescence chemistry.Results:SP cells with stem cell potency were isolated from GBC-SD cells with a proportion of(0.64±0.08)%.The metastatic ability of SP cells was obviously higher than that of non-SP cells and GBC-SD cells(P<0.05).The expression of ABCG2 was significantly higher in SP cells than in non-SP cells and GBC-SD cells[(89.56±3.86)%vs.(1.32±0.49)%and(12.37±1.61)%,P=0.001].The expression of Oct-4 in these cells was(94.87±1.40)%,(88.16±2.34)%and(90.17±1.61)%,respectively(P>0.05).CD34 was neady absent in these cells on protein level[(1.78±0.51)%vs.(0.63±0.21)%and(0.96±0.381)%,P>0.05)],but it was highly expressed in non-SP cells and GBC-SD cells and absent in SP cells off mRNA leve;.Conclusion:SP calls which hava the potency of stem cells,exist in human gallbladder carcinoma GBC-SD cell line and have the phenotype of ABCG2+Oct-4+CD34-.