Effect of Autologous Tumor Cell Lysate-loaded Dendritic Cell Vaccine in Combination with Cytokine-induced Killer Cells on Hepatocellular Carcinoma
10.3969/j.issn.1000-8179.2009.24.008
- VernacularTitle:抗原致敏DC联合CIK在原发性肝癌治疗中的应用
- Author:
Guocheng ZHONG
;
Xiaoyu ZHANG
;
Yi SUN
;
Hong KUANG
;
Xinrong JING
;
Min MIN
;
Hao LIAO
;
Jian CHEN
- Publication Type:Journal Article
- Keywords:
Primary hepatocellular carcinoma;
Dendritic cells;
Cytokine-induced killer cells;
Tumor immunity
- From:
Chinese Journal of Clinical Oncology
2009;36(24):1404-1408
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the killing activity of cytokine-induced killer (CIK) cells after incubation with autologous tumor cell lysate-pulsed dendritic cells (Ag-DC) and to evaluate the immune functions of patients, the clinical efficacy and side effect of Ag-DC in combination with CIK on hepatocellular carcinoma. Methods: Peripheral blood mononuclear cells were isolated from 24 patients with hepatocellular carcinoma, and cultured with granulocyte-macrophage colony-stimulating factor and interleukin-4 to produce dendritic cells (DCs). The DCs were pulsed with autologous tumor cell lysate. T lymphocytes from PBMC were cultured with interferon-Y (IFN-γ), IL-2, CD3-moAb, and IL-1α to prepare CIK. The killing effect of CIK on SMMC-7721 was investigated after CIK was incubated with Ag-DC. After immunotherapy with Ag-DC and CIK, immunolog-ic and clinical responses of the 24 patients were evaluated. Results: The killing effect of CIK was remarkably improved after CIK was incubated with Ag-DC. The immunotherapy with DC and CIK alleviated symptoms and improved the immune functions of the patients. Except for transient fever and chill, no remarkable adverse events were observed. Conclusion: Ag-DC in combination with CIK shows short-term efficacy on hepatocellular carcinoma through inducing specific anti-tumor immunity and can be an effective adjuvant therapy for hepatocellular carcinoma.
