The Effect of Pioglitazone on the Expression of Thrombospondin-1 in the Kidney of Diabetic Rats
10.3870/j.issn.1672-0741.2009.06.002
- VernacularTitle:吡格列酮对糖尿病大鼠肾组织TSP1表达的影响
- Author:
Ling FU
;
Tian DAI
;
Jianshe LIU
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
pioglitazone;
thrombospondin-1;
transforming growth factor-β1
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):725-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pioglitazone on the expression of thrombospondin-1(TSP1)and transfor ming growth factor-β1 (TGF-131)in the kidney of diabetic rats.Methods Forty Sprague-Dawley(SD)rats were randomly divided into 4 groups:normal control(NC),streptozocin-induced diabetic mellitus(DM),diabetic rats treated with pioglitazone(DP)and normal control rats treated with pioglitazone(NP).24-h urine protein quantity,serum creatinine(Scr) and kidney hypertrophy index were measured after treatment for 10 weeks,and the expression of TSP1 and TGF-β1 in the kidney was detected by using RT-PCR and immuohistochemistry.Results As compared with DM group,urine protein level and kidney hypertrophy index were significantly attenuated in DP group(P<0.05),but there was no significant difference in plasma glucose between them(P>0.05).As compared with NC and NP groups,the expression of TSP1 and TGF-β1 was significantly elevated in the kidney of DM and DP groups(P<0.01).Both TSP1 and TGF-β1 expression in DP group was lower than in DM group(P<0.05).Conclusion Pioglitazone can exert a direct protective effect on the kidney in diabetic rats,which is independent of the role of insulin sensitizer,and the mechanism may be associated with the decreased TSP1 expression in the kidney.
