Inhibitory Effects of Glucocorticoids on the Tenascin C Expression in Nasal Polyp
10.3870/j.issn.1672-0741.2009.06.009
- VernacularTitle:糖皮质激素对鼻息肉中固生蛋白C表达的抑制作
- Author:
Heng WANG
;
Zheng LIU
;
Xiang LU
;
Xuejun YOU
;
Qixue GAO
;
Yonghua CUI
- Publication Type:Journal Article
- Keywords:
glucocorticoids;
nasal polyps;
epithelial cells;
tenascin C;
transforming growth factor-β1
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):756-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of glucocortieoids on tenasein C(TNC) expression in nasal polyp tissues and airway epithelia,and explore the possible mechanism of glucocorticoids inhibiting remodeling of nasal polyp tissue.Methods The enzyme linked immunosorbent assay(ELISA) was used to detect the protein levels of tenascin C and transforming growth factor-β1(TGF-β1) in nasal polyp tissues from intranasal glucocorticoids (Budesonide,BUD) treated group and untreated group.The cell culture,real-time RT-PCR and in situ ELISA were employed to investigate the regulatory effects of budesonide on the TNC mRNA and protein expression in BEAS-2B immortalized human bronchial epithelial cells.Results The protein levels of TNC and TGF-β1 were decreased in nasal polyp tissues from intranasal BUD-treated group as compared with untreated group(P<0.01).There was a significantly positive correlation between TNC and TGF-β1 protein levels in nasal polyp tissues (r =0.68,P<0.01).After preincubation with BUD,TNC mRNA and protein expression induced by TGF-β1 in BEAS-2B cells was significantly decreased(P<0.01).Conclusion Glucocorticoids might participate in the regulation of tissue remodeling in nasal polyp by inhibiting the TGF-β1 expression in nasal polyp tissue and suppressing the induction of TGF-β1 to up-regulatie the TNC expression in airway epithelia.
