Effects of Propofol on the Heme Oxygenase-1 Expression in Rats with H_2O_2-mediated Damage to Cultured Cardiac Myocytes
10.3870/j.issn.1672-0741.2009.06.010
- VernacularTitle:异丙酚对大鼠心肌细胞氧化损伤时血红素加氧酶-1表达的影响
- Author:
Jinjin XU
;
Long WANG
- Publication Type:Journal Article
- Keywords:
propofol;
hydrogen peroxide;
heine oxygenase-1;
cardiac myocytes
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):760-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of propofol on the heme oxygenase-1(HO-1)expression in rats with H_2O_2-mediated damage to cultured cardiac myocytes.Methods Primary cultured new-born rat cardiac myocytes were divided into 8 groups:control,H_2O_2 group,low dose propofol (LP) group,middle dose propofol(MP)group,high dose propofol(HP)group,LP +ZnPP Ⅸ group.MP+ZnPP Ⅸ group and HP+ZnPP Ⅸ group.The cells in all groups were incubated for 6 h.The concentration of malondialdehyde(MDA),activity of superoxid dismuase(SOD),mitochondria and Caspase-3,and the expression of HO-1 were determined.Results As compared with H_2O_2 group,the HO-1 expression,and the activity of SOD and mitochondria were significantly increased(P<0.05,P<0.01),and activity of Caspase-3 and concentration of MDA was decreased(P<0.05)in MP and HP groups.As compared with LP,MP and HP groups respectively.the HO-1 expression and SOD activity were significantly decreased and the concentration of MDA increased in LP+ZnPP IX,MP+ZnPP Ⅸ and HP+ZnPP Ⅸ groups(P<0.05,P<0.01),while Caspase-3 activity was significantly increased in MP+ZnPP IX and HP+ZnPP IX(P<0.05,P<0.01).Conclusion Propofol can protect cardiac myocytes against H_2O_2-mediated cytotoxicity in a dose-dependent manner,and increase the HO-1 expression,which may partly mediate cytoprotective effects of propofol.
