The Analysis of the HP1-γ Expression in Different Grades of Esophageal Carcinoma by Using Laser Scanning Cytometer
10.3870/j.issn.1672-0741.2009.06.011
- VernacularTitle:应用激光扫描细胞仪分析食管癌中HP1-γ蛋白的表达
- Author:
Wei LI
;
Hong DONG
;
Jia LIU
;
Shuang LI
;
Wei WANG
;
Ding MA
- Publication Type:Journal Article
- Keywords:
heterochro-Matin-associated protein 1;
esophageal carcinoma laser scanning eytometer tissue microarray
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):764-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the heterochro-Matin-associated protein 1(HP1-γ) expression during the carcinogenesis and progresston of esophageal carcmoma,and preliminarily investigate the supertortty of using laser scanning cytometer to analyze immunohistochemistry results compared to traditional scoring methods.Methods A tissue microarray containing different grades of esophageal carcinoma was selected and the HP1-γ expression was detected by immunohistochemistry.The results of immunohistochemistry were analyzed quantitatively by using laser scanning cytometer.The correlation of results analyzed by using laser scanning cytometer and traditional scoring methods was analyzed by chi square test.Results The HP1-γ was primarily expressed in the nucleus.The positive rate of HP1-γ in normal esophagus,moderate-severe atypical hyperplasia，in situ carcinoma and squamous cancer was 37.5% (3/8),100%(21/21),100%(7/7) and 23.7% (9/38),respectively,with the difference being statistically significant among normal esophagus，oderate-severe atypical hyperplasia plus in situ carcinoma and squamous cancer(P<0.01).There was a high correlation between the results analyzed by laser scanning cytometer and those by traditional scoring methods under a light microscope(P<0.01).Conclusion HP1-γ may play a resistant role in the transformation from normal esophageal cells to malignant cells.Compared to the traditionally artificial scoring methods,there are many advantages such as high resolution,high objectivity and accuracy when using laser scanning cytometer to analyze the immunohisto chemistry results.
