Preparation of I-1-P Chitosan-antisense Nanoparticles and Their Inhibition against M.tuberculosis Growth
10.3870/j.issn.1672-0741.2009.06.013
- VernacularTitle:I-1-P反义ODN-壳聚糖纳米粒子的制备及对结核杆菌生长的抑制作用
- Author:
Jie SHEN
;
Zhifei CHEN
;
Hongling ZHANG
;
Xiaobo LI
;
Shi LU
;
Yuanyuan LI
- Publication Type:Journal Article
- Keywords:
chitosan;
I-1-P antisense ODN;
combined nanoparticle;
M.tuberculosis
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):771-774
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of applying nanotechnology against M.tuberculosis growth.Methods Chitosan-antisense ODN nanoparticles were prepared by complex coacervation method.Varied amount of ODNs and chitosanODN nanoparticles were added to the cultures and the growth of the bacilli was monitored.Results The nanoparticles were composed of(35.6±0.9)% ODN and(64.4±0.9)% chitosan.Compared to the free ODN,antisense nanopartilces were more effective in inhibiting the proliferation of M.tuberculosis.Antisense nanoparticles decreased growth by(2.8±0.1)CFU/ml at concentration of 4/μmol/L.Conclusion Chitosan-ODN nanoparticles were more effective in inhibiting the growth of M.tuberculosis than free ODN.
