Protective Effect of DADLE on Pulmonary Function in Rats with Sepsis
10.3870/j.issn.1672-0741.2009.06.019
- VernacularTitle:δ阿片受体激动剂对脓毒症大鼠肺功能的保护作用
- Author:
Chengwu TANG
;
Ying BAO
;
Ming ZHU
;
Xiaohong WEN
;
Mouyun FEI
;
Xiaolan ZHANG
;
Xiaoyi JIANG
;
Yao WANG
;
Wenming FENG
- Publication Type:Journal Article
- Keywords:
delta opioid receptor;
DADLE;
sepsis;
cecal ligation and puncture
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2009;38(6):796-799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effects of DADLE on pulmonary function in rats with sepsis and the mechanism.Methods Seventy-two SD rats were randomly divided into sham operated group(SO),septic group(SEP),and DADLEtreated group (DADLE).Sepsis model was induced by cecal ligation and puncture(CLP).In SO group,the abdomen was opened without any other treatment.In DADLE-treated group,DADLE(0.5 mg/ml) was administerd at a dose of 5 ml/kg by intravenous injection after CLP.Rats were sacrificed at the 2nd,6th and 10th h after CLP.Arterial blood was collected for blood gas analysis.The wet-to-dry lung weight ratio was measured,and the levels of TNF-a and IL-6 in blood were detected.The levels of MPO,MDA and ATP in lung tissue were determined.The pathologic changes of the lungs were examined under a light microscope.Results As compared with SEP group,PaCO_2,and PaO_2 were significantly increased(P<0.05),while the wet-to-dry ratio and levels of serum TNF-a and IL-6 were significantly reduced(P<0.05),and levels of MPO and MDA were significantly reduced(P<0.05) and content of ATP in lung tissue was significantly increased (P<0.05)in DADLE group.More severe pathological changes were found in SEP group than in DADLE group.Conclusion DADLE has pulmonary protective effects in rats with sepsis.
