Survivin-siRNA inhibits proliferation of lung cancer A549 cells and enhances their chemosensitity to cisplatin
10.3872/j.issn.1007-385X.2009.06.006
- VernacularTitle:Survivin-siRNA抑制肺癌A549细胞的增殖并增强其对顺铂的敏感性
- Author:
Shuguang ZHANG
;
Xiaofan LIU
;
Jiang DU
;
Wenya LI
;
Lin ZHANG
- Publication Type:Journal Article
- Keywords:
survivin;
RNA inference;
lung neoplasms;
A549 cell;
cisplatin
- From:
Chinese Journal of Cancer Biotherapy
2009;16(6):583-587
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of survivin-siRNA plasmid on survivin expression in human lung cancer cell line A549, and to observe its effect on the apoptosis, proliferation, and chemosensitivity of A549 cells. Methods: pSilencer-survivin-siRNA (survivin-siRNA) plasmid was constructed using pSilencer-U6 plasmid and was transfected into A549 cells. Expression of survivin mRNA and protein was examined by RT-PCR and Western blotting analysis, respec-tively. Apoptosis and proliferation of A549 cells were examined by DAPI staining and MTT, respectively. Results: Sur-vivin-siRNA plasmid was successfully constructed, and it significantly inhibited survivin mRNA and protein expression in A549 cells. Survivin-siRNA transfection induced apoptosis, inhibited proliferation and increased chemosensitivity of A549 cells to cisplatin. Conclusion: pSilencer-survivin-siRNA can silence survivin expression in A549 cells and subsequently inhibit proliferation, promote apoptosis, and enhance chemosensitivity of A549 cells to cisplatin. Survivin may serve as a potential target for gene therapy of lung cancer.
