MucA mutation and its alginate-production in clinically isolated Pseudomonas aeruginosa
- VernacularTitle:临床分离铜绿假单胞菌mucA基因突变与藻酸盐合成的关系
- Author:
Jie MENG
;
Chengping HU
;
Bailing LUO
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
biofilm;
mucA mutation;
alginate
- From:
Journal of Central South University(Medical Sciences)
2009;34(12):1196-1201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the characterization of mucA gene mutation in clinically isolated Pseudomonas aeruginosa (P.aeruginosa), and the relation between mucA mutation and the mucoid phenotype.Methods A total of 58 strains of P.aeruginosa were collected. Of them,8 were nonmucoid phenotype and 50 were mucoid phenotype.We detected mucA mutations with PCR-SSCP and sequencing analysis. Alginate was examined by colorimetry. Results All strrains had mucA mutations (100%), 16 of the 50 (32%) isolates contained mucA mutations that could alter the encoding sequence of amino acids, and the rate in nonmucoid isolates was 0. Fourteen mutation sites were found, 5 of which could alter the encoding sequence of amino acids, and the others were silent mutations. The alginate concentration of mucoid P.aeruginosa was higher than the nonmucoid P.aeruginos(P＜0.01). The alginate concentration of the isolates which contained mucA mutations that could alter the encoding sequence of amino acid was higher than the strains only with silent mutations (P＜0.01).Conclusion mucA mutation correlates with the alginate production and phenotype of bacterial colonies.
