Analyses of Phenolic Compounds of Water Extract from Propolis by Reversed Phase High Performance Liquid Chromatography
- VernacularTitle:反相高效液相色谱法测定蜂胶水提物中的酚类化合物
- Author:
Bin CHEN
;
Liping LUO
;
Yan LI
;
Yuanjun XU
;
Yuxin FU
;
Yinyu GAO
- Publication Type:Journal Article
- Keywords:
Reversed phase high performance liquid chromatography;
propolis;
water extract;
phenolic component
- From:
Chinese Journal of Analytical Chemistry
2009;37(12):1786-1790
- CountryChina
- Language:Chinese
-
Abstract:
A reversed phase high-performance liquid chromatography method for the simultaneous analysis of 23 kinds of phenolic components in water extract of porpolis (WEP) was developed. The separation was performed on ZORBAX Eclipse XDB C_(18) column (150 mm×4.6 mm, 5 μm) by gradient elution. The mobile phase consisted of ethanol with 0.1% formic acid at the flow rate of 1.0 mL/min. The detection wavelengths were 256 and 280 nm. The injection volume was 20 μL, and the column temperature was maintained at 35 ℃. The method showed good linear relationship, precision and repeatability. The recoveries were between 93.3% and 106.6%. Eighteen Reference compounds were detected in WEP of Hehei by this method. The content of Catechin was the highest(30.50 mg/g), the next was 3,4-dimethoxycinnamic acid (15.41 mg/g). Nine Reference compounds were detected in WEP of Yunnan. The content of catechin was also the highest (11.23 mg/g), the next was chrysin (15.41 mg/g). Similarity of WEPs between the products of Hebei and Yunnan were 0.099 (256 nm) and 0.194 (280 nm). The chemical compositions of two WEPs were remarkably different.
