Biodistribution and Scintigraphy of Iodine-131-Iododeoxyadenosine in Rats Bearing Breast Cancer.
- Author:
Seon Gu KIM
;
Chang Guhn KIM
;
Kang Mo LEE
;
Hye Won KIM
;
Byung Cheol MIN
;
See Sung CHOI
;
Jong Deuk LEE
;
David J YANG
;
E Edmund KIM
;
Hyun Chul LEE
;
Jong Jin WON
- Publication Type:Original Article
- Keywords:
lododeoxyadenosine;
Biodistribution;
Autoradiography;
Tumor scintigraphy;
Breast cancer
- MeSH:
Adenine;
Animals;
Autoradiography;
Breast Neoplasms*;
Breast*;
DNA;
Female;
Heart;
Hot Temperature;
Humans;
Intestines;
Kidney;
Liver;
Lung;
Radionuclide Imaging*;
Rats*;
Spleen;
Thyroid Gland;
X-Ray Film
- From:Korean Journal of Nuclear Medicine
1998;32(4):374-381
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: I-131 labeled (2'-deoxy-2-iodo-p-D-arabinofuranosyl) adenine (IAD) may be involved in DNA synthesis during active proliferation of tumor cells. We conducted this study to find out the biodistribution of IAD and its feasibility for scintigraphic tumor imaging. MATERIALS AND METHODS: Tosyl acetyl-adenosine was dissolved in acetonitrile, and I-131-NaI was added and heated to synthesize IAD. Female Fisher 344 rats innoculated with breast tumor cells were injected witb 0.27 MBq of IAD. Rats were sacrificed at 0.5, 1, 2, 4, 24h and the % of injected dose per gram of tissue (%ID/g) was determined. For scintigraphy, rats bearing breast cancer were administered with 1.11 MBq of IAD and imaging was perforrned after 2 and 24h. Then, rat body was fixed and rnicrotomized slice was placed on radiographic film for autoradiography, RESULTS: %ID/g of tumor wa.' 0.74 (0.5h), 0.73 (1h), 0.55 (2h), 0.38 (4h), and 0.05 (24h), respectively. At 1h after injection, %ID/g of tumor was higher than that of heart (0.34), liver (0.42), spleen (0.47), kidney (0,69), muscle (0.14), bone (0.33) and intestine (0.51). However, %1D/g of tumor was lower than blood (1.06), lung (0.77), and thyroid (177.71). At 4h, %ID/g of tumor in comparison with other tissue did not change. Tumor contrast expressed by tumor to blood ratio was 0.69 and tumor to muscle ratio was 5.11 at 1h. However, these ratios did not improve through 24h. On autoradiogram and scintigraphy at 2 and 24 hour, the tumor was well visualized. CONCLUSION: This results suggest that Ial) may have a potential for tumor scintigraphy. However, further work is needed to improve localization in tumor tissue.
