Effect of recombination human activin A on proliferation and apoptosis of A549
- VernacularTitle:重组人activin A对A549细胞增殖及凋亡的影响
- Author:
Baiding WANG
;
Yunye NING
;
Yulin FENG
;
Fuqiang WEN
- Publication Type:Journal Article
- Keywords:
activin A;
A549;
MTT assay;
flow cytometry;
type Ⅱ receptors of activin
- From:China Oncology
2009;19(12):894-898
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: It was reported that activins, members of transforming growth factor-β superfamily, could induce several tumor cells into apoptosis. This study was designed to observe the effects of recombination human activin A on proliferation and apoptosis ofA549 cells. Methods: A549 cells were cultivated by routine method, then treated with different concentrations of recombination human activin A. Inhibitory effect of activin A on A549 cell proliferation was detected by MTT assay, and apoptosis of A549 cells was determined by flow cytometry. The expression of type Ⅱ receptors of activin (ActR Ⅱ and ActR Ⅱ B) was detected by Western blot. Results: Recombination human activin A inhibited the proliferation ofA549 cells in time-dependent and dose-dependent manners. Increases of apoptosis in A549 cells and expression of ActR I] and ActR ⅡB occurred dose-dependently after treated with acfivin A. Conclusion: Recombination human activin A inhibited cell proliferation and induced apoptosis in A549 cells, at least in part, by inducing expression of type Ⅱ receptors of activin and activation of its downstream signaling.
