Biocompatibility of rat olfactory ensheathing cells and fibrin glue
10.3969/j.issn.1673-8225.2009.47.009
- VernacularTitle:纤维蛋白凝胶与大鼠嗅鞘细胞的生物相容性
- Author:
Guanhua XU
;
Zhiming CUI
;
Zhen HUANG
;
Min SUN
;
Weidong LI
;
Guofeng BAO
;
Yuyu SUN
;
Lingling WANG
;
Leyin ZHU
;
Ying CUI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(47):9249-9252
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Fibrin glue has been demonstrated to function as a kind of biomaterial with high quality. It has been used in nerve tissue engineering and proved to be a kind of scaffold for some cells.OBJECTIVE: To explore the biocompatibility of fibrin glue and olfactory ensheathing cells (OECs).DESIGN, TIME AND SETTING: An in vitro control trial based on cytology was performed at the Institute of Neurobiology,Nantong University from August 2007 to February 2008.MATERIALS: Fibrin glue was made of fibrin and catalyst, and OECs derived from rats' olfactory bulb were normally primary-cultured.METHODS: OECs were divided into control (OECs clone spheres were cultured alone) and in fibrin glue (OECs clone spheres were cultured and combined with fibrin glue) groups. After 1 week of culture, the proliferation of OECs were observed by convert microscope and detected by S-100 immunofluorescence histochemical staining.MAIN OUTCOME M EASURES: OECs morphology, cell count, the area of the cell bodies and the perimeter of the cell were determined.RESULTS: OECs could survive, migrate in fibrin glue, and float in the fibrin glue in the lower layer. After 7 days of incubation, cell body exhibited fusiform or triangle, predominantly bipolar or bipolar. The number of the S-100 positive cells was more, and cell bodies were larger in fibrin glue group than control group (P < 0.05). However, there was no obvious difference between two groups in cell perimeter (P > 0.05).CONCLUSION: Fibrin glue has good biocompatibility with OECs, and OECs can survive and migrate in fibrin glue.
