Local injection of embryonic neural stem cells for treatment of experimental spinal cord injury: Evaluation of somatosensory and motor evoked potentials and hind limb motor function
10.3969/j.issn.1673-8225.2009.49.005
- VernacularTitle:胎鼠神经干细胞局部注射移植大鼠高位脊髓损伤缺损处:体感及运动诱发电位与后肢运动功能评价
- Author:
Guangzhi WANG
;
Mingna LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(49):9637-9640
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: To improve neural regeneration and functional recovery following spinal cord injury (SCI) remains difficult. Embryonic neural stem cells benefit neuronal survival and promote axonal regeneration.OBJECTIVE: To explore the feasibility of the local injection of embryonic neural stem cells to treat rats with high level SCI, and evaluate its effect by neuroelectrophysiology and motor function of hind limbs.DESIGN, TIME AND SETTING: In vivo experiment of cytology was performed at the Animal Experimental Center of Harbin Medical University from June 2007 to June 2008.MATERIALS: A total of 40 healthy adult SD rats were randomly divided into two groups (n=20): normal saline and cell transplantation groups. In addition, 5 SD rats, pregnant 14 days, were used for preparation of embryonic neural stem cells.METHODS: High level SCI was made in two groups. Briefly, 2 cm of bilateral intercostal nerves respectively from the 8~(th) to 10~(th) costal bones was crossing implanted in the SCI (proximal white matter and distal gray matter; distal white matter and proximal gray matter). Cell transplantation group was locally injected with 2×10~6 embryonic neural stem cells, and normal saline group was injected with the same volume of normal saline.MAIN OUTCOME MEASURES: Somatosensory evoked potential (SEP) and motor evoked potentials (MEP) testing, observation of the recovery of neuroelectrophysiology; BDA anterograde neural tracer; the recovery of motor conduction by the main beam;BBB hind limb motor function score.RESULTS: SEP and MEP latency and amplitude of cell transplantation group were better than normal saline group (P < 0.01).BDA-positive nerve fibers through the tag were found in cell transplantation group, but no in normal saline group. BBB hind limb motor function score of cell transplantation group was significantly improved compared with normal saline group improved (P < 0.01).CONCLUSION: Local injection of embryonic neural stem cells betters neuroelectrophysiology and motor function of hind limbs following high level SCI.
