Osteogenesis and angiogenesis of tissue-engineered biomimetic periosteum constructed by combination of small intestinal submucosa matrix with bone marrow mesenchymal stem cells in rabbits
10.3969/j.issn.1673-8225.2009.51.015
- VernacularTitle:以小肠黏膜下层基质为支架复合骨髓间充质干细胞构建组织工程仿生骨膜:在兔体内的成骨及其血管化
- Author:
Lin ZHAO
;
Shuanke WANG
;
Ping DONG
;
Junsheng WANG
;
Jia LIU
;
Junlong HAO
;
Meng WU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(51):10079-10082
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the in vivo possibility of osteogenesis and angiogenesis of tissue-engineered periosteum in rabbits.METHODS: The marrow mesenchymal stem cells (MSCs) derived from New Zealand rabbits were adhered to small intestinal submucosa (SIS) to fabricate the tissue-engineered periosteum. Totally 12 New Zealand rabbits were received critical bone defect in bilateral radii to prepare models. The tissue-engineered periosteum was randomly implanted in one side of bone defect,and the other side was treated by SIS. At 4 weeks after operation, the angiogenesis of tissue engineered bone was detected by Tetracycline fluorescence microscopy and formaldehyde-ink perfusion method; simultaneously, the new bone formation was firmed by haematoxylin-eosin staining.RESULTS: Animals showed normal daily behaviors and non-infection wounds healing. The gross observation showed that bone defects in the experimental side were bridged with newly formed bone; while the defects of the control side were remained empty.Tetracycline fluorescence microscopy and hisotological examination could confirm the new bone tissue formation in the experimental side. The ink staining in new bone specimens suggested that there were abundant of neovasculization in tissue-engineered bone.CONCLUSION: Tissue-engineered periosteum can form new bone in allogenic rabbits and can be vascularized by some inherent mechanism for new bone tissue survivor.
