Effects of recombinant human bone morphogenetic protein 2 on integrin beta 1 expression during mandibular distraction
10.3969/j.issn.1673-8225.2009.50.003
- VernacularTitle:重组人骨形成蛋白2对下颌骨牵引成骨中整合素β_1表达的影响
- Author:
Luming TIAN
;
Zhiying WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2009;13(50):9818-9821
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Integfin levels can affect cellular function,regulate bone formation and bone resorption.However,this process need activated by other factors,whether recombinant human bone morphogenetic protein 2 (rhBMP-2)has this effect is poorly understood.OBJECTIVE:To study the effects of the different concentration of rhBMP-2 on the expression of inergrin β_1 during the mandibular dfScraction in rabbits.DESIGN,TIME AND SETTING:The randomized,controlled.animal grouping experiment of histological observation was performed at the Animal ExperimentaI Center and Immunohistochemistry Center of Liaoning Medical University from May 2006 to May 2007.MATERIALS:A total of 45 Japanese white rabbits were used.rhBMP-2 was supplied by Beijing Academy of Military Medical Sciences.METHODS:The rabbits were prepared for mandibular distraction models and randomly assigned into control,1.5 mg rhBMP-2 and 3.0 mg rhBMP-2 groups.with 15 animals in each group.Collagen sponge and collagen binding proteins were implanted into mandibles of rabbits,respectively,and distraction 0.4 mm per time,twice per day for 5 days.The mandibles were lengthened 4 mm.MAIN OUTCOME MEASURES:The expression of integrin 131 was detected using immunohistochemistry at days 1,3,7,14,and 28 after distraction.RESULTS:All rabbits were included in the final analysis.The integdn β_1 mainly expressed at 7 days after distraction.At the same time point.when concenrtation rhBMP-2 was increased,the positive integrin β_1 rate and positive stained area percentage were increased(P<0.05).Compared to the same concentration,the positive integrin β_1 rate and positive stained area percentage were gradually increased as time prolonged (P<0.05).CONCLUSION:The integdn β_1 expression can be promoted by rhBMP-2 at the anaphase of mandibular distraction osteogenesis in a concentration-dependent manner.
