Amperometric Glucose Biosensor Based on Carbon Nanoparticles
- VernacularTitle:基于Nafion/碳纳米粒子修饰的葡萄糖传感器
- Author:
Meifang WANG
;
Wei ZHANG
;
Bin FANG
- Publication Type:Journal Article
- Keywords:
Carbon nanoparticles;
Hydrogen peroxide peroxide;
Glucose;
Electrochemical biosensor
- From:
Chinese Journal of Analytical Chemistry
2010;38(1):125-128
- CountryChina
- Language:Chinese
-
Abstract:
The nafion/carbon nanoparticles (CNPs) composite film modified glassy carbon electrode was pre pared by dropwise method, and the electrocatalyst of H_2O_2 at Nafion/CNPs modified electrode was investiga ted.The results show that electrocataytic activitie for detection of H_2O_2 at the modified electrode is very good.The biosensing application of CNPs was demonstrated through fabrication of an electrochemical biosensor.The biosensor was constructed by encapsulating glucose oxidase in the Nafion/CNPs composite film.The biosensor had good electrocatalytic activity toward oxidation of glucose.The glucose biosensor shows a linear range from 2.0 × 10 ~(-6) to 6.0×10~(-3) mol/L with a detection limit of 1.6×10 ~(-6) mol/L.The biosensor shows high stabili ty, good reproducibility and can avoid the commonly coexisted interference.In addition, real rat serum samples were analyzed by this biosensor with satisfactory results.