Application of Electrical Driven and Cationic Exchange Membrane to Capture and Enrich Deoxyribonucleic Acid
- VernacularTitle:电驱动与阳离子交换膜在脱氧核糖核酸捕获浓缩中的应用
- Author:
Huabin HUANG
;
Zhixia ZHUANG
;
Wenhua ZHANG
;
Jia HU
;
Chaoyong YANG
;
Xiaoru WANG
- Publication Type:Journal Article
- Keywords:
Deoxyribonucleic acid capture;
Electrical driven;
Ion-exchange membrane;
Concentration
- From:
Chinese Journal of Analytical Chemistry
2010;38(1):143-146
- CountryChina
- Language:Chinese
-
Abstract:
Based on the selective permeability of cationic exchange membrane and the characteristic of electri cal driven, a new and non-damage apparatus that can be used to separate compounds with different electric property was built to capture and enrich DNA.λ-DNA, as a model compound, was used to study the charac teristic of capturing and enriching DNA in this device.Factors that might affect the efficiency such as voltage and flow speed were optimized and gel electrophoresis was done to study if DNA was denaturation, PCR was done to prove the concentration can be used to further bioanalysis.Results were got; the apparatus could used to capture and enrich DNA scathelessly under the condition of low-voltage, the concentrate multiple was 62, and the recovery efficiency was accessible to 47%, concentration can be used for the next bioanalysis.Auto matic, easy to handle and little organic reagent using made it be advantageous, it could be miniatured to com bine with other biochips.This method had a large application space in field of DNA extraction, purification and concentration.
