Effect of 12(S)-HETE on the p27~(kip1) expression in rat mesangial cells and glomeruli
- VernacularTitle:12(S)-HETE对大鼠系膜细胞和肾小球内p27~(kip1)表达的影响
- Author:
Yingchun CUI
;
Jie ZHANG
;
Qingxin LIU
;
Qiaoyan GUO
;
Man WU
;
Dongmei ZHANG
;
Lining MIAO
;
Zhonggao XU
- Publication Type:Journal Article
- Keywords:
12(S)-HETE;
p27~(kip1);
Mesangial cell;
Glomeruli
- From:
Chinese Journal of Immunology
2009;25(12):1067-1069
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of 12(S)-HETE on the p27~(kip1) expression in mesangial cells and glomeruli.Methods:Mesangial cells were exposed to 12(S)-HETE.12(S)-HETE was infused to rats by osmotic mini-pump.Total protein content measurement for cell hypertrophy,RT-PCR for mRNA expression and Western blot for protein expression were performed respectively.Results:12(S)-HETE stimulation induced mesangial cell hypertrophy and p27~(kip1) protein expression,but not p27~(kip1) mRNA expression.Furthermore,p27~(kip1) mRNA and protein expression in the glomeruli were significantly increased by 12(S)-HETE stimulation using osmotic mini-pump.Conclusion:12(S)-HETE plays an important role in the pathogenesis of glomerular cell hypertrophy and senescence through upregulation of p27~(kip1) expression.
