Effect of engineered heterogeneous skins cells on phenotypes of langerhans cells
- VernacularTitle:组织工程皮肤细胞对朗格汉斯细胞表型的影响
- Author:
Jianjun BI
;
Jinjin WU
;
Yadong YANG
;
Tangyou ZHU
;
Caimao ZHANG
- Publication Type:Journal Article
- Keywords:
Tissue engineered skin;
Keratinocyte;
Fibroblast;
Langerhans cells
- From:
Chinese Journal of Immunology
2009;25(12):1100-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of seeding cells of tissue-engineered skins,keratinocytes and fibroblasts,on the phenotypes of langerhans cells (LC).Methods:Peripheral blood mononuclear cells were induced by cytokines,granulocyte macrophage-colony stimulating factor (GM-CSF),interleukin 4 (IL-4),transforming growth factor β1 (TGF-β1) to generate LCs,which were subsequently cocultured with allogenic keratinocytes or fibroblasts.Then the phenotypes of langerhans cells was detected by flow cytometer (FCM),and the degree of proliferation of autogeneic lymphocytes was assessed after stimulation with LCs.Results:Cultured LCs had low level expression of HLA-DR,CD80,CD86 and no expression of CD83.Cocultured with allogenic keratinocytes or fibroblasts,phenotypes of LCs did not change significantly.Simultaneously,LCs could not stimulate autogeneic lymphocytes proliferation.Conclusion:These data indicate that LCs cocultured with seeding cells of tissue-engineered skins remain immature state.It is suggested that seeding cells of tissue-engineered skins have low immunogenicity and are unable to induce significant immunological rejection of host after transplantation.
