The effect of mTOR/S6K1 signaling pathway on the development of high fat diet-induced mouse insulin resistance
10.3969/j.issn.1006-6187.2009.12.002
- VernacularTitle:mTOR/S6K1信号通路在高脂饮食诱导小鼠胰岛素抵抗发生中的作用
- Author:
Hong YUAN
;
Yanmei NIU
;
Yanhui LIU
;
Zhaopeng SU
;
Huige LI
;
Li FU
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
mTOR;
Effector
- From:
Chinese Journal of Diabetes
2009;17(12):884-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of mTOR/S6K1 signaling pathway on the development of insulin resistantce. Methods 20 male C57BL/6 mice were divided into normal diet group (NC) and high fat diet group (HF).HF mice were fed with high fat diet for 14 weeks and insulin resistance was confirmed in all mice. We observed the morphology of pancreatic islet by HE staining. Serum insulin concentration was also evaluated by ELISA. Northern blot, Western blot and immunofluorescence were performed to detect mTOR and S6K1 mRNA and protein expression in skeletal muscle. Results As compared with NC group,HF group showed that the body weight and fasting serum insulin level were increased by 21.99%(P＜0.05) and 181.82%(P＜0.01) respectively;the area of pancreatic islet was significantly increased;glucose tolerance was impaired;expressions of mTOR mRNA (125.61±10.43 vs 100.00, P＜0.05) and protein (137.41±7.86 vs 100.00, P＜0.01) were significantly increased. And we also found an significant increase in total S6K1 mRNA (154.98±16.26 vs 100.00, P＜0.01) and protein (137.36±3.08 vs 100.00,P＜0.01) as well as pS6K1 protein (390.15±69.62 vs 50.59±16.65,P＜0.01)expression in HF group as compared with NC group.Conclusions mTOR/S6K1 signaling pathway plays an important role in the development of higt fat diet induced insulin resistance.
